| Name |
Bleomycin Sulfate
Blenoxane |
| CAS# |
9041-93-4 (bleomycin sulfate) |
| CAS# |
11056-06-7 (bleomycin) |
| NSC# |
125066 (bleomycins) |
| Category |
Peptide antibiotic, DNA strand scission agent. |
| Binding Mode |
Intercalation |
| Site Specificity |
5'-GpT-3' and 5'-GpC-3' [Takeshita, 1978] |
| Effect on DNA |
DNA unwinding angle 12o, lengthens DNA 0.31 nm per drug
insertion [Povirk, 1979]. Alters PAGE migration of intrinsically
bent DNA [Nightingale, 1992]. |
| Source |
Blenoxane is a mixture of bleomycins, approximately 60% bleomycin A2,
30% bleomycin B2 and 10% other bleomycins.[Burger, 1985]. Natural
product produced by Streptomyces verticillus. |
| Preparation and Use |
Extinction coefficient of 1.7x104 M-1 cm-1 at 291 nm [Burger, 1985].
Repeated use degrades activity. Requires coordinated metal ions (eg,
Fe-II) for strand scission activity [Burger, 1981]. Stock can be
dissolved in ddH2O. |
