| Name |
DAPI
4',6-diamidino-2-phenylindole dihydrochloride hydrate |
| MW |
350.3 (Boehringer-Mannheim) |
| CAS# |
23541-50-6 |
| Category |
Intercalator/Minor Groove Binder |
| Binding Mode |
Minor groove binding at low drug concentration as per x-ray crystal
structure [Kopka, 1985]. Intercalation at higher concentration [Wilson,
1990] |
| Site Specificity |
A/T preference at low concentration, 3-4 bp site by DNase I footprinting
[Portugal, 1988], in agreement with crystal structure [Kopka, 1985]; G/C
at higher concentration. |
| Effect on DNA |
At higher drug concentrations, intercalative binding results in DNA
unwinding |
| Preparation and Use |
Prepare in ddH2O |
