| Name |
Doxorubicin.HCll |
| MW |
579.99 (Aldrich) |
| CAS# |
25316-40-9 |
| NCS# |
123127 |
| Category |
Anthracycline |
| Binding Mode |
Intercalation, amino sugar directed into minor groove [Frederick, 1990]. |
| Site Specificity |
3-bp binding site, highest preference for two adjacent G/C base pairs
flanked by an A/T base pair [Chaires, 1987; Frederick, 1990]. Studies
using stoppage of in vitro transcription show a preference for 5'-(T)CA-3'
sites [Trist, 1989] |
| Effect on DNA |
Unwinding angle of 11o [Waring, 1981]. Negligible
distortion of helical conformation [Frederick, 1990]. |
| Preparation and Use |
Dissolved in DMSO. Light sensitive drug. UV Absorbance:
1.12x104 M-1 cm-1 at 480nm [Barcelo, 1988].
General handling considerations are reviewed in [Bosanquet, 1986] |
