| Name |
Echinomycin
Quinomycin A |
| MW |
1101.27 [Budvari, 1989] |
| CAS# |
512-64-1 |
| NCS# |
526417 |
| Category |
Bicyclic peptide antibiotic |
| Binding Mode |
Bisintercalation. Solution [Gilbert, 1991] and crystal structures
[Quigley, 1986] indicate that echinomycin positions the CpG step between
chromophores; peptide bridge binds in the minor groove. |
| Site Specificity |
The van Dyke footprinting study defines the selectivity rule as:
5'-W(usually C)G(T>G>C,A)-3' [van Dyke, 1984]. Every echinomycin
footprint contains CpG steps [Low, 1984; Fox, 1990].
A weak, secondary interaction has been demonstrated with ApT steps, but
the interaction cannot be footprinted with DNase I. [Fox, 1991] |
| Effect on DNA |
Unwinding angle of 48o [Waring, 1981]. Bases adjacent
to CpG may be Hoogstein paired [Quigley, 1986 ; Gao, 1988; Ughetto, 1985].
May unbend statically bent DNA [Cons, 1990]. |
| Preparation and Use |
Dissolved in DMSO, yielding clear liquid. Poorly soluble in water.
Extinction coefficient of 1.15x104 M-1 cm-1
at 325 nm [Waring, 1974] |
