| Name |
Hedamycin |
| MW |
748.7 [Schmidtz, 1966] |
| CAS# |
11048-97-8 |
| NCS# |
70929F5, 70929D |
| Category |
Pluramycin |
| Binding Mode |
Threading intercalation and covalent bond formation to N7-G.
Possible threading mode. [Sun, 1993]. Anthrapyran intercalated, C2
diepoxide is positioned in major groove,C8 and C10 sugars are in minor
groove [Sun, 1995; Hansen, 1995]. |
| Site Specificity |
Intercalates 5' to guanine which is alkylated [Bennett, 1982].
Studies examining heat-labile sites proposed consensus sites of 5'-TG-3'
[Sun, 1993]; 5'-TGT-3' and 5'-CGT-3' [Murray, 1995], and most recently
5'-N(C>T)*G(G>A,T). Adducts to N7-G [Sun, 1995]. |
| Source |
Obtained from Bristol-Myers, Lot w87g19. Yellow powder, provided
in glass container. Originally isolated from Streptomyces griseoruber
C-1150 [Schmidtz, 1966 #311] |
| Preparation and Use |
Prepared by adding 1/10th final volume 0.1N HCl, adding 8/10 vol ddH2O,
and adding 1/10th vol NaOH to neutralize. Prior to adding NaOH, solution
is yellow, but turns purple when neutralized . Drug decomposes in
basic solution [Schmidtz, 1966] or with exposure to light [Fredenhagen,
1985]. Extinction coefficient of 1.00x104 M-1
cm-1 at 428 nm. [White, 1969]. |
