| Name |
Neocarzinostatin
Zinostatin |
| CAS# |
9014-02-2 |
| NCS# |
69856 |
| MW |
Apoprotein 10,700 (tightly associated non-protein chromophore MW ~659)
[Meienhofer, 1972; Ishida, 1965]. |
| Category |
Enediyne-containing peptide antibiotic |
| Binding Mode |
Intercalation of napthoate moiety [Povirk, 1981] |
| Site Specificity |
Adducted bases occur T>A>>C>G [Poon, 1977; Golik, 1987], oligopyrimidine
runs cleaved preferentially [Sugiura, 1989]. UV-activated ncs has
increased activity against G [Uesawa, 1989]. |
| Effect on DNA |
Strand scission agent, activated by reducing agents or UV irradiation.
Intercalation unwinds DNA by 21o, each molecule lengthens DNA
by 0.33 nm [Povirk, 1981]. |
| Source |
Originally extracted from Streptomyces carzinostaticus var.
F-41 [Ishida, 1965]. |
| Preparation and Use |
200 microM stock prepared in ddH2O |
