| Name |
U-77,779
Bizelesin |
| MW |
815.72 |
| Category |
Cyclopropylpyrroloindole |
| Binding Mode |
Minor groove binding drug, forms covalent adduct to N3-A. |
| Site Specificity |
Crosslinks adenines on opposite strands of DNA, 6 to 7 bp apart.
Requires an A/T sequence, with a preference for runs of A [Lee, 1993].
In a favorable context, one end can adduct to guanine [Sun, 1993]. |
| Effect on DNA |
Eliminates intrinsic bending of A/T-rich regions [Lee, 1993], stabilizes
DNA in an unbent conformation [Thompson, 1995]. |
| Preparation and Use |
Initial stock are prepared from powder, 2mg/mL in N,N dimethylacetamide.
Substock diluted to 2.5mM with DMSO. Further dilutions are prepared
with water. |
