| Name | Chromomycin A3
Toyomycin Aburamycin B |
| CAS# | 7059-24-7 |
| MW | 1183.3 (Sigma) |
| Category | Aureolic acid |
| Binding Mode | Minor groove binder. Demonstrated to bind certain sequences as dimer by NMR [Gao, 1989; Gao, 1990], and quantitative DNase I footprinting [Dabrowiak, 1989] |
| Site Specificity | G/C sequences, usually at least 3 consecutive G/C as determined by footprinting studies [van Dyke, 1983; Fox, 1985; Cons, 1989] and in vitro binding studies with oligonucleotides [Liu, 1994] |
| Effect on DNA | Dimer binding results in wide, shallow minor groove with major groove compression [Gao, 1989]. One report states that DMS methylation pattern is altered [Fox, 1985], but a conflicting report also exists [Dabrowiak, 1989] |
| Source | Originally isolated from Streptomyces griseus |
| Preparation and Use | Yellow powder, provided in glass container. Prepared in EtOH or DMSO, using extinction 39,800 M-1 cm-1 at 282 nm [van Dyke, 1983], 10,000 M-1 cm-1 at 406 nm [Liu, 1994]. Soluble in DMSO and EtOH. Requires Mg+2 for DNA binding activity [Ward, 1965]. |
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