| Name | cis-Platinum
Platinol cis-diamminedichloroplatinum (II) cis-DDP |
| MW | 300.05 [Budvari, 1989] |
| CAS# | 15663-27-1 |
| NCS# | 119875 |
| Category | Synthetic organometallic complex. |
| Binding Mode | Formation of covalent adduct; capable of bidentate binding, forming
intra- or interstrand crosslinks.
|
| Site Specificity | Bonds reactive sites of guanine (and to much lesser extent, adenine) in the major groove. The most reactive site is N7-G. Most common products found in natural DNAs are distributed approximately as: 65% cis-GG, 25% cis-AG, 10% cis-GNG, monofunctional adducts and interstrand crosslinks [Bellon, 1991; Eastman, 1986; Fichtinger-Shepman, 1985]. |
| Preparation and Use | Provided as a white powder, then dissolved in sterile, isotonic saline. Prior to usage, stock is thawed, warmed to 37oC in water bath, and alternately vortex and sonicated into solution. Dilutions are prepared immediately and used. Limited solubility in water (0.253g/100g at 25oC) [Budvari, 1989]. Extinction coefficient at 260nm is 6,600 M-1 cm-1 [Fichtinger-Shepman, 1982]. |
| DNA drugs |
| Heme-Onc |
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